Journal: EMBO Molecular Medicine

Article Title: Impaired migration and lung invasion of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75 NTR

doi: 10.1038/s44321-025-00297-1

Figure Lengend Snippet: ( A ) Western blot analysis of cleaved PARP in A875 melanoma cells in response to increasing concentrations (0.5 to 5 µM) of Div17E5 and Np75-4A22. A potent inducer of apoptosis, anisomycin (1 μM), was used as positive control. Reprobing for GAPDH was used as loading control. The histogram on the right shows quantification expressed as average of three independent experiments, each performed in triplicate ± SEM. ( B ) Time course of wound healing in A875 control (NT) and knock-down (shp75) cells in response to low micromolar and submicromolar concentrations of Div17E5 (left side) or Np75-4A22 (right side). Results are plotted as mean ± SEM ( N = 3). * P = 0.035 (one-way ANOVA followed by Tukey’s multiple comparisons). ( C ) Boyden chamber analysis of A875 cell chemotaxis in response to NGF (50 ng/ml) in the presence or absence or Div17E5 (left side graph) or Np75-4A22 (right side graph). Results are plotted as mean ± SEM ( N = 3 independent experiments each performed in triplicate). # P = 0.0465 vs. no NGF; * P = 0.0435 vs. no drug (one-way ANOVA followed by Tukey’s multiple comparisons). ( D ) Boyden chamber analysis of A875 cell chemotaxis in response to NGF (50 ng/ml) in the presence of submicromolar concentrations of Np75-4A22. Results are plotted as mean ± SEM ( N = 2 independent experiments each performed in triplicate). # P = 0.0477 vs. no NGF; * P = 0.0462 and ** P = 0.0078 vs. NGF without drug (one-way ANOVA followed by Tukey’s multiple comparisons). ( E ) Boyden chamber analysis of WM115 cell chemotaxis in response to NGF (100 ng/ml) in the presence of Np75-4A22 (1 μM). Results are plotted as mean ± SEM ( N = 3 independent experiments each performed in triplicate). ### P = 0.0004 vs. vehicle; *** P = 0.0004 vs. NGF without drug (one-way ANOVA followed by Tukey’s multiple comparisons). .

Article Snippet: WM115 cells ( H. sapiens ) , ATCC , CRL-1675.

Techniques: Western Blot, Positive Control, Control, Knockdown, Chemotaxis Assay